Detalls de la publicació.

Article

Any:2019
Autor(s):J.M. Valencia, A. Grau, T. Pretto, J. Pons, J.A. Jurado-Rivera, J.A. Castro, A. Toffan, G. Catanese
Títol:Viral encephalopathy and retinopathy (VER) disease in Epinephelus marginatus from the Balearic Islands marine protected areas
Revista:DISEASES OF AQUATIC ORGANISMS
ISSN:0177-5103
JCR Impact Factor:1.368
Volum:135
Pàgines:49-58
D.O.I.:https://doi.org/10.3354/dao03378
Web:https://www.int-res.com/abstracts/dao/v135/n1/p49-58/
Resum:his is the first description of a betanodavirus infection in the dusky grouper Epi-nephelus marginatuswithin the marine protected areas (MPAs) of the Balearic Islands.Histopathology techniques were employed to describe neurological lesions in infected fish.Abnormal swimming, mortality, and neurological lesions were detected in all analysed grouperindividuals. Virus particles were observed by means of transmission electron microscopy. Reversetranscription of RNA1 and RNA2 followed by cDNA amplification and sequencing allowed viralclassification. Phylogenetic analysis showed the isolates from wild E. marginatusof the BalearicIslands MPAs to be closely related to Dicentrarchus labraxand Mullus barbatusstrains fromCyprus and Italy. Although vertical transmission from infected spawners has been described asthe major route for nodavirus infection, we point out in this work that horizontal transmissionamong sub-clinical fishes after migration or commercial import for aquaculture production couldplay a major role in the spreading of the disease in MPA

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